Deane D L, Harvey E, Steel C M
Medical Research Council, Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.
Clin Exp Immunol. 1991 Jan;83(1):175-81. doi: 10.1111/j.1365-2249.1991.tb05610.x.
We have examined the effect of monoclonal antibodies (MoAbs) to different epitopes of the leucocyte common antigen (LCA), CD45, on anti-human immunoglobulin-primed B cell activation. Binding of MoAbs to restricted epitopes present on CD45 glycoproteins of 180 kD and 220 kD (designated CD45R0 and CD45R, respectively) was found to promote B cell proliferation in the presence of T cells. CD45 MoAbs reactive with 'public' determinants on all four constituent members of the LCA family (180, 190, 205, and 220 kD) had either little effect or inhibited the basal B cell response to anti-immunoglobulin priming. Simultaneous immunofluorescent analysis of 5-bromodeoxyuridine incorporation and the expression of CD19 (B cell specific) or CD2 (T cell specific) identified the majority of responder cells as B lymphocytes. CD45R MoAbs significantly enhanced the B cell response to sub-optimal concentrations of interleukin-2. CD45 and CD45R0 MoAbs failed to elicit a similar response. Antibody to the interleukin-2 receptor (anti-Tac) partially blocked the CD45R-driven, T cell-dependent B cell proliferation.
我们研究了针对白细胞共同抗原（LCA）即CD45不同表位的单克隆抗体（MoAbs）对经抗人免疫球蛋白致敏的B细胞活化的影响。发现与180 kD和220 kD的CD45糖蛋白上存在的受限表位（分别命名为CD45R0和CD45R）结合的MoAbs在有T细胞存在的情况下可促进B细胞增殖。与LCA家族所有四个组成成员（180、190、205和220 kD）上的“公共”决定簇反应的CD45 MoAbs对基础B细胞对抗免疫球蛋白致敏的反应影响很小或有抑制作用。对5-溴脱氧尿苷掺入以及CD19（B细胞特异性）或CD2（T细胞特异性）表达的同步免疫荧光分析确定大多数反应细胞为B淋巴细胞。CD45R MoAbs显著增强了B细胞对次优浓度白细胞介素-2的反应。CD45和CD45R0 MoAbs未能引发类似反应。白细胞介素-2受体抗体（抗-Tac）部分阻断了CD45R驱动的、T细胞依赖性的B细胞增殖。
