Rothschild M F, Chen H L, Christian L L, Lie W R, Venier L, Cooper M, Briggs C, Warner C M
J Anim Sci. 1984 Sep;59(3):643-9. doi: 10.2527/jas1984.593643x.
Genetic differences in immune response to B. bronchiseptica after vaccination with a commercial B. bronchiseptica bacterin were investigated in 1,069 8-wk-old pigs. These pigs were from 65 litters born in the spring and 66 litters born in the fall of 1982 and were purebreds from the Chester White (n = 128), Duroc (n = 281), Hampshire (n = 143), Landrace (n = 309) and Yorkshire (n = 208) breeds. Each litter was raised separately. Individual pigs were vaccinated im at 4 and 6 wk of age with 2 ml of B. bronchiseptica bacterin. At 8 wk of age, 8 ml of blood were collected from each animal and serum prepared to determine agglutinating antibody titers against B. bronchiseptica bacterin by a bacterial agglutination method. In addition, lymphocytes were separated from 1 ml of heparinized blood and used to determine Swine Lymphocyte Antigen (SLA) haplotypes by using cytotoxic antibodies against the SLA complex. Antisera for 3 SLA haplotypes were made available by the National Institutes of Health. Results indicated that breed of pig (P less than .01) and dam of pig (P less than .01) affected the immune response of the pig after B. bronchiseptica vaccination. Higher immune response was also associated (P less than .05) with one of the SLA haplotypes tested. Heritability estimates for immune response following vaccination were .10 +/- .12 (half-sib) and .42 +/- .19 (full-sib). Results suggest that the relationship of the SLA complex to immune response in the pig and nonadditive genetic and maternal effects on immune response should be further investigated.
在1069头8周龄仔猪中研究了接种市售支气管败血波氏杆菌菌苗后对该菌免疫反应的遗传差异。这些仔猪来自1982年春季出生的65窝和秋季出生的66窝，是切斯特白猪（n = 128）、杜洛克猪（n = 281）、汉普夏猪（n = 143）、长白猪（n = 309）和约克夏猪（n = 208）的纯种。每窝仔猪分开饲养。个体仔猪在4周龄和6周龄时肌肉注射2毫升支气管败血波氏杆菌菌苗。在8周龄时，从每头动物采集8毫升血液并制备血清，通过细菌凝集法测定针对支气管败血波氏杆菌菌苗的凝集抗体滴度。此外，从1毫升肝素化血液中分离淋巴细胞，并用针对SLA复合体的细胞毒性抗体来确定猪淋巴细胞抗原（SLA）单倍型。国立卫生研究院提供了针对3种SLA单倍型的抗血清。结果表明，猪的品种（P <.01）和猪的母亲（P <.01）影响支气管败血波氏杆菌疫苗接种后猪的免疫反应。较高的免疫反应也与所测试的一种SLA单倍型相关（P <.05）。接种疫苗后免疫反应的遗传力估计值为0.10±0.12（半同胞）和0.42±0.19（全同胞）。结果表明，应进一步研究SLA复合体与猪免疫反应的关系以及非加性遗传和母体对免疫反应的影响。
