Brunette D M
J Cell Sci. 1984 Jul;69:35-45. doi: 10.1242/jcs.69.1.35.
The influence of mechanical stretching on epithelial (E) cells was examined by culturing E cells derived from the epithelial cell rests of Malassez on a flexible plastic substrate and stretching the substrate by means of an orthodontic screw. A significant increase in the number of E cells synthesizing DNA was observed after just 30 min of stretching. In 17 experiments the ratio of cells labelled with tritiated thymidine in cultures stretched for 2 h to the number of labelled cells in unstretched controls was 1.92 +/- 0.34. An increase in labelling as a result of stretching was found for E cells cultured at either high or low cell-population densities but the effect was most pronounced for E cells cultured at higher concentrations of foetal bovine serum. Morphometric analysis of electron micrographs of stretched and unstretched cultures indicated that the stretched cultures had a higher volume fraction of filamentous structures and more desmosomes per unit length of cell membrane than unstretched cultures. The behaviour of E cells in response to stretching in vitro appears to be similar to the response of the epithelial rests in vivo when the latter are exposed to tension as a result of forces produced by orthodontic techniques.
通过将源自马拉色氏上皮剩余细胞的上皮（E）细胞培养在柔性塑料基质上，并借助正畸螺钉拉伸该基质，研究了机械拉伸对上皮（E）细胞的影响。拉伸仅30分钟后，就观察到合成DNA的E细胞数量显著增加。在17个实验中，拉伸2小时的培养物中用氚标记胸腺嘧啶核苷标记的细胞与未拉伸对照中标记细胞数量的比率为1.92±0.34。在高或低细胞群体密度下培养的E细胞均发现由于拉伸导致标记增加，但在较高浓度胎牛血清中培养的E细胞中这种效应最为明显。对拉伸和未拉伸培养物的电子显微镜照片进行形态计量分析表明，与未拉伸培养物相比，拉伸培养物具有更高的丝状结构体积分数和每单位细胞膜长度更多的桥粒。体外E细胞对拉伸的反应行为似乎与体内上皮剩余细胞在因正畸技术产生的力而受到张力时的反应相似。
