Hasegawa S, Sato S, Saito S, Suzuki Y, Brunette D M
Calcif Tissue Int. 1985 Jul;37(4):431-6. doi: 10.1007/BF02553714.
A simple method was devised for applying mechanical stretching to bone cell cultures. Bone cells cultured on the flexible plastic membrane of a Petriperm dish are placed over a template with a convex surface. A lead weight is then placed on top of the dish which causes the membrane and the tightly attached cells to be stretched. Mechanical stretching, applied either intermittently or continuously for a 2-hour period resulted in a 64% increase in the number of cells synthesizing DNA. Stretching the cells also significantly increased incorporation of tritiated proline and tritiated leucine. To assay the ratio of collagenous to noncollagenous protein, medium and cell layers of cultures labeled with tritiated leucine were incubated with collagenase and the digests chromatographed on PD 10 columns. The amount of collagen synthesized by stretched and unstretched cultures did not differ; but an increased synthesis of noncollagenous proteins was observed in the stretched cultures.
设计了一种对骨细胞培养物施加机械拉伸的简单方法。培养在皮氏培养皿柔性塑料膜上的骨细胞被放置在具有凸面的模板上。然后在培养皿顶部放置一个铅锤，这会导致膜和紧密附着的细胞被拉伸。间歇性或连续施加2小时的机械拉伸导致合成DNA的细胞数量增加了64%。拉伸细胞还显著增加了氚化脯氨酸和氚化亮氨酸的掺入。为了测定胶原蛋白与非胶原蛋白的比例，用氚化亮氨酸标记的培养物的培养基和细胞层与胶原酶一起孵育，并将消化物在PD 10柱上进行色谱分析。拉伸和未拉伸培养物合成的胶原蛋白量没有差异；但在拉伸培养物中观察到非胶原蛋白的合成增加。
