Bowden R A, McGavren L, Hayward A R, Levin M J
J Clin Microbiol. 1984 Oct;20(4):696-700. doi: 10.1128/jcm.20.4.696-700.1984.
Methods are described for obtaining and growing fibroblasts from bone marrow for use as virus-infected targets. Fibroblasts obtained at the time of routine marrow examination were maintained through 12 to 18 passages as confluent monolayers. Bone marrow fibroblasts could be infected with varicella-zoster virus, and these infected cells were suitable targets for a 51Cr-release cytotoxicity assay. Since these virus-infected cells retain their HLA-A and -B antigens, they are readily available to study the immune cells which mediate virus-specific cytotoxicity.
描述了从骨髓中获取并培养成纤维细胞以用作病毒感染靶标的方法。在常规骨髓检查时获得的成纤维细胞作为汇合单层细胞维持传代12至18次。骨髓成纤维细胞可被水痘 - 带状疱疹病毒感染，并且这些感染的细胞是51Cr释放细胞毒性测定的合适靶标。由于这些病毒感染的细胞保留其HLA - A和 - B抗原，它们很容易用于研究介导病毒特异性细胞毒性的免疫细胞。
