Nagao T, Komatsuda M, Yamauchi K, Arimori S
J Cell Physiol. 1981 Aug;108(2):155-61. doi: 10.1002/jcp.1041080206.
In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus fibroblast colony growth. Human bone marrow cells are plated as 2 x 10(5) cells in the culture. Normal human bone marrow yields 47 +/- 4 fibroblasts colonies per 2 x 10(5) cells plated. Bone marrow fibroblast cultures using agar or methylcellulose restrict colony formation. Marked colony suppression was observed in acute leukemia, and a discrete colony number was observed in hypoplastic anemia. This fibroblast culture method should be applied to a larger number of patients to determine whether it has a pathognomonic value and clinical significance.
在人骨髓的液体培养中，成纤维细胞集落于第6至9天形成。使用20%的胎牛血清作为刺激成纤维细胞集落生长的物质。将人骨髓细胞以2×10⁵个细胞接种到培养物中。正常的人骨髓每接种2×10⁵个细胞可产生47±4个成纤维细胞集落。使用琼脂或甲基纤维素的骨髓成纤维细胞培养会限制集落形成。在急性白血病中观察到明显的集落抑制，而在再生障碍性贫血中观察到离散的集落数。这种成纤维细胞培养方法应应用于更多患者，以确定其是否具有诊断价值和临床意义。
