Meck J M, Goldberg E H
J Immunol Methods. 1984 Oct 26;73(2):293-9. doi: 10.1016/0022-1759(84)90404-6.
A sensitive and reliable serological assay for the detection of H-Y antigen is described which uses monoclonal H-Y antibody and a cellular radioimmunobinding assay on cultured human fibroblasts. Cell lines from 2 normal human females, 2 normal human males and one XX human male were tested in 3 separate assays. Cells from XY and XX males were found to contain H-Y antigen; however, the reaction against the XX male cells was found to be intermediate between the XY male and normal XX female cells.
描述了一种用于检测H-Y抗原的灵敏且可靠的血清学检测方法，该方法使用单克隆H-Y抗体并在培养的人成纤维细胞上进行细胞放射免疫结合检测。在3次独立检测中对来自2名正常人类女性、2名正常人类男性和1名XX男性的细胞系进行了检测。发现XY男性和XX男性的细胞含有H-Y抗原；然而，发现针对XX男性细胞的反应介于XY男性细胞和正常XX女性细胞之间。
