Nagamine C, Reidy J, Koo G C
Transplantation. 1984 Jan;37(1):13-7. doi: 10.1097/00007890-198401000-00006.
Radiobinding assays using monoclonal H-Y antibodies were performed on human peripheral blood leukocytes, concanavalin-A-(conA)3 stimulated blast cells, and fibroblast primary cell cultures. For peripheral blood leukocytes, male-specific binding was demonstrated only when the cells were enriched for T cells and the H-Y antisera were partially purified for the IgG or IgM fractions. Similar results were obtained with con-A-stimulated blast cells. Fibroblast cells could be tested with unpurified H-Y antisera. With our monoclonal antibodies, best results were obtained using con A blast cells and fibroblasts.
利用单克隆H-Y抗体对人外周血白细胞、伴刀豆球蛋白A（ConA）刺激的母细胞以及成纤维细胞原代细胞培养物进行放射结合试验。对于外周血白细胞，仅当细胞富含T细胞且H-Y抗血清针对IgG或IgM组分进行部分纯化时，才证明存在雄性特异性结合。用ConA刺激的母细胞也得到了类似结果。成纤维细胞可用未纯化的H-Y抗血清进行检测。使用我们的单克隆抗体，用ConA母细胞和成纤维细胞可获得最佳结果。
