一种具有抗α-半乳糖基特异性的独特天然人IgG抗体。
A unique natural human IgG antibody with anti-alpha-galactosyl specificity.
作者信息
Galili U, Rachmilewitz E A, Peleg A, Flechner I
出版信息
J Exp Med. 1984 Nov 1;160(5):1519-31. doi: 10.1084/jem.160.5.1519.
A new natural anti-alpha-galactosyl IgG antibody (anti-Gal) was found to be present in high titer in the serum of every normal individual studied. The antibody was isolated by affinity chromatography on a melibiose-Sepharose column. The reactivity of the antibody was assessed by its interaction with alpha-galactosyl residues on rabbit erythrocytes (RabRBC). The specificity was determined by inhibition experiments with various carbohydrates. The anti-Gal interacts with alpha-galactosyl residues, possibly on glycolipids of human RBC (HuRBC), after removal of membrane proteins by treatment with pronase. In addition, the anti-Gal bind specifically to normal and pathologically senescent HuRBC, suggesting a physiological role for this natural antibody in the aging of RBC. The ubiquitous presence of anti-Gal in high titers throughout life implies a constant antigenic stimulation. In addition to the theoretical interest in the antibody, the study of the anti-Gal reactivity seems to bear immunodiagnostic significance. Decrease in the antibody titer was found to reflect humoral immunodeficiency disorders.
研究发现，在每个被研究的正常个体血清中，一种新的天然抗α-半乳糖基IgG抗体（抗Gal）呈高滴度存在。该抗体通过在蜜二糖-琼脂糖柱上进行亲和层析分离得到。通过其与兔红细胞（RabRBC）上的α-半乳糖基残基的相互作用来评估该抗体的反应性。通过用各种碳水化合物进行抑制实验来确定其特异性。在用链霉蛋白酶处理去除膜蛋白后，抗Gal与可能存在于人红细胞（HuRBC）糖脂上的α-半乳糖基残基相互作用。此外，抗Gal特异性结合正常和病理性衰老的HuRBC，这表明这种天然抗体在红细胞衰老过程中具有生理作用。抗Gal在一生中普遍以高滴度存在，这意味着持续的抗原刺激。除了对该抗体的理论研究兴趣外，对抗Gal反应性的研究似乎具有免疫诊断意义。发现抗体滴度降低反映体液免疫缺陷疾病。
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