Walum E, Peterson A
J Toxicol Environ Health. 1984;13(4-6):511-20. doi: 10.1080/15287398409530516.
The acute toxic action of a number of common chemicals was tested by their ability to cause detachment of cultured mouse neuroblastoma C1300 cells. A TD25 value was obtained by graphic estimation of the concentration needed to cause 25% of the total cell number to detach. These TD25 values were compared with LD50 values obtained from the literature, and they were found to correlate with a coefficient of 0.86. For six of the tested substances-diuron, butylated hydroxytoluene, benzidine, cyclophosphamide, Na2SeO3, and KCN-a very poor correlation was obtained. These diverging results could be ascribed to deficiencies in the neuroblastoma cell detachment test and emphasize the necessity for combined in vitro test procedures.
通过一些常见化学物质使培养的小鼠神经母细胞瘤C1300细胞脱离的能力，测试了它们的急性毒性作用。通过图形估计使25%的总细胞数脱离所需的浓度，获得了TD25值。将这些TD25值与从文献中获得的LD50值进行比较，发现它们的相关系数为0.86。对于六种受试物质——敌草隆、丁基化羟基甲苯、联苯胺、环磷酰胺、亚硒酸钠和氰化钾——相关性非常差。这些不同的结果可能归因于神经母细胞瘤细胞脱离试验的缺陷，并强调了联合体外试验程序的必要性。
