Låg M, Paulsen G, Jonsen J
J Toxicol Environ Health. 1984;13(4-6):857-64. doi: 10.1080/15287398409530546.
Trachea from albino mice were cut transversely into nearly identical rings and incubated in medium 199 with Hanks salts and HEPES buffer at 37 degrees C. Sodium selenite at 0.5-5 mM depressed the ciliary activity. With 1 and 5 mM sodium selenite, a 50% reduction in the activity index was observed after approximately 5 and 1.5 h, respectively. The ATP content in trachea rings was reduced with 0.05-5 mM sodium selenite, and increasing concentrations gave decreasing amount of ATP after incubation for 4 and 21 h. The rate of protein synthesis as determined by incorporation of radioactive leucine was reduced with 0.5 and 2 mM sodium selenite. The synthesis was reduced quickly by 2 mM sodium selenite, which gave a 30% reduction after incubation for 1 h. It seems that the ATP levels may be used as the most sensitive indication of sodium selenite toxicity in mouse trachea.
将白化小鼠的气管横向切成几乎相同的环状物，并在含有汉克斯盐和HEPES缓冲液的199培养基中于37℃孵育。0.5 - 5 mM的亚硒酸钠会抑制纤毛活性。使用1 mM和5 mM亚硒酸钠时，分别在约5小时和1.5小时后观察到活性指数降低了50%。0.05 - 5 mM的亚硒酸钠会使气管环中的ATP含量降低，孵育4小时和21小时后，随着浓度增加，ATP含量减少。通过放射性亮氨酸掺入测定的蛋白质合成速率在0.5 mM和2 mM亚硒酸钠作用下降低。2 mM亚硒酸钠使合成迅速降低，孵育1小时后降低了30%。看来ATP水平可能是亚硒酸钠对小鼠气管毒性最敏感的指标。
