Greene S A, Wolff R K, Hahn F F, Henderson R F, Mauderly J L, Lundgren D L
J Toxicol Environ Health. 1984;13(4-6):945-58. doi: 10.1080/15287398409530552.
This study was done to produce a model of chronic bronchitis. Twelve beagle dogs were exposed to 500 ppm sulfur dioxide (SO2) for 2 h/d, 5 d/wk for 21 wk and 4 dogs were sham-exposed to filtered ambient air for the same period. Exposure effects were evaluated by periodically examining the dogs using chest radiographs, pulmonary function, tracheal mucous clearance; and the cellular and soluble components of bronchopulmonary lavage fluids. Dogs were serially sacrificed after 13 and 21 wk of exposure and after 6 and 14 wk of recovery. Clinical signs produced in the SO2-exposed dogs included mucoid nasal discharge, productive cough, moist rales on auscultation, tonsilitis, and conjunctivitis. Chest radiographs revealed mild peribronchiolar thickening. Histopathology, tracheal mucous clearance measurements, and lavage cytology were consistent with a diagnosis of chronic bronchitis. It is concluded that repeated exposure to 500 ppm SO2 for 21 wk produced chronic bronchitis in the beagle dog. Complete recovery occurred within 5 wk following cessation of SO2 exposure.
本研究旨在建立慢性支气管炎模型。12只比格犬每天暴露于500 ppm二氧化硫（SO₂）中2小时，每周5天，持续21周；4只犬在同一时期接受过滤后的环境空气假暴露。通过定期使用胸部X光片、肺功能、气管黏液清除率以及支气管肺泡灌洗液体的细胞和可溶性成分对犬进行检查来评估暴露效果。在暴露13周和21周后以及恢复6周和14周后依次对犬实施安乐死。暴露于SO₂的犬所产生的临床症状包括黏液性鼻分泌物、湿性咳嗽、听诊时有湿啰音、扁桃体炎和结膜炎。胸部X光片显示轻度支气管周围增厚。组织病理学、气管黏液清除率测量和灌洗细胞学检查结果均符合慢性支气管炎的诊断。得出的结论是，比格犬反复暴露于500 ppm SO₂中21周会导致慢性支气管炎。在停止SO₂暴露后的5周内完全恢复。
