Funnell W R
J Neurosci Methods. 1984 Aug;11(3):205-10. doi: 10.1016/0165-0270(84)90040-2.
This paper concerns certain aspects of the calculation of the surface area of objects reconstructed from serial sections. The main points discussed are (1) the relative importance of the number of sections compared to the number of segments taken around the contour in each section; and (2) the fact that the error in the estimated surface area may actually become worse as the number of sections increases. The number of sections should be made large enough to reproduce the three-dimensional shape of the object, but not too much larger; the number of segments around the contours should then be made large enough that the segment size is similar to or smaller than the section thickness.
本文涉及从连续切片重建的物体表面积计算的某些方面。讨论的要点是：（1）与每个切片中围绕轮廓所取的片段数量相比，切片数量的相对重要性；以及（2）随着切片数量增加，估计表面积的误差实际上可能会变得更糟这一事实。切片数量应足够多以再现物体的三维形状，但不宜过多；然后围绕轮廓的片段数量应足够多，以使片段大小与切片厚度相似或小于切片厚度。
