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用于解剖学研究的连续显微切片成像与建模

Imaging and modelling from serial microscopic sections for the study of anatomy.

作者信息

Durikovic R, Kaneda K, Yamashita H

机构信息

Electric Machinery Laboratory, Faculty of Engineering, Hiroshima University, Japan.

出版信息

Med Biol Eng Comput. 1998 May;36(3):276-84. doi: 10.1007/BF02522471.

DOI:10.1007/BF02522471
PMID:9747565
Abstract

A system is considered for segmenting noisy intensity images and consequent three-dimensional object reconstruction from a set of planar contours. A new semi-automatic method for the extraction of contours from a sequence of cross-sectional images based on an active contour model (ACM) is proposed. The dynamic ACM proceeds along the sequence of cross-sections following a non-rigid motion, in accordance with the organ boundary. Image texture information is also employed in the model. Problems associated with topological reconstruction from planar contours are addressed, and several criteria promoting semi-automatic topological reconstruction are introduced. The proposed system is successfully applied to the processing of real data related to animal embryonic organs, proving that the system allows detailed modelling of irregular objects. The reconstructed models can be observed in wire-frame, solid, transparent or stereoscopic semi-transparent format. The human-computer interaction implemented in the procedure assists with problems of feature identification and object manipulation about an arbitrary axis.

摘要

本文考虑了一种用于分割噪声强度图像并从一组平面轮廓进行三维物体重建的系统。提出了一种基于主动轮廓模型(ACM)从一系列横截面图像中提取轮廓的新半自动方法。动态ACM根据器官边界,沿着横截面序列进行非刚性运动。该模型还利用了图像纹理信息。解决了与从平面轮廓进行拓扑重建相关的问题,并引入了一些促进半自动拓扑重建的标准。所提出的系统成功应用于与动物胚胎器官相关的真实数据处理,证明该系统允许对不规则物体进行详细建模。重建模型可以以线框、实体、透明或立体半透明格式进行观察。该过程中实现的人机交互有助于解决特征识别和围绕任意轴的物体操纵问题。

相似文献

1
Imaging and modelling from serial microscopic sections for the study of anatomy.用于解剖学研究的连续显微切片成像与建模
Med Biol Eng Comput. 1998 May;36(3):276-84. doi: 10.1007/BF02522471.
2
On the calculation of surface areas of objects reconstructed from serial sections.关于从连续切片重建的物体表面积的计算。
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本文引用的文献

1
Computer-assisted registration, segmentation, and 3D reconstruction from images of neuronal tissue sections.基于神经元组织切片图像的计算机辅助配准、分割和 3D 重建。
IEEE Trans Med Imaging. 1994;13(2):351-62. doi: 10.1109/42.293928.
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Three-dimensional computer modelling system for the study of biological structures.用于生物结构研究的三维计算机建模系统。
Med Biol Eng Comput. 1993 Nov;31(6):557-61. doi: 10.1007/BF02441801.
3
Computerized three-dimensional reconstructions of serial sections in electron microscopy.电子显微镜下连续切片的计算机三维重建
Ultramicroscopy. 1990 Jan;32(1):48-54. doi: 10.1016/0304-3991(90)90093-2.
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A new method for three-dimensional reconstruction from serial sections by computer graphics using "meta-balls": reconstruction of "hepatoskeletal system" formed by Ito cells in the cod liver.一种利用“元球”通过计算机图形学从连续切片进行三维重建的新方法:鳕鱼肝脏中由伊托细胞形成的“肝骨骼系统”的重建。
Comput Biomed Res. 1990 Feb;23(1):37-45. doi: 10.1016/0010-4809(90)90005-w.
5
CARTOS II semi-automated nerve tracing: three-dimensional reconstruction from serial section micrographs.
Comput Med Imaging Graph. 1990 Sep-Oct;14(5):319-29. doi: 10.1016/0895-6111(90)90106-l.