Yanase T, Nawata H, Higuchi K, Kato K, Ibayashi H
Life Sci. 1984 Oct 29;35(18):1869-75. doi: 10.1016/0024-3205(84)90538-1.
The effect of dexamethasone on dispersed cells in primary monolayer culture from bovine adrenal medulla and human extramedullary pheochromocytoma was examined by estimating the level of catecholamines (CAs) and Methionine-enkephalin (Met-enk) in the medium and cells. In cultured bovine adrenal chromaffin cells, dexamethasone caused significant increase in Met-enk levels 18 hours after administration. There was no release of Met-enk and CAs in the medium 10 min after administration, although nicotine did cause a significant release of Met-enk and CAs. A dose response increase in the level of CAs and Met-enk in bovine adrenal chromaffin cells was obtained with doses varying between 0 and 10(-6)M dexamethasone 18 hours after administration. In cultured human extramedullary pheochromocytoma cells, dexamethasone significantly increased the levels of norepinephrine and Met-enk in a dose dependent manner 24 hours after administration. These results suggest that dexamethasone does not act as a secretagogue but may be related to the synthesis of Met-enk and CAs.
通过评估培养基和细胞中儿茶酚胺（CAs）和甲硫氨酸脑啡肽（Met-enk）的水平，研究了地塞米松对牛肾上腺髓质原代单层培养的分散细胞和人髓外嗜铬细胞瘤的影响。在培养的牛肾上腺嗜铬细胞中，地塞米松给药18小时后导致Met-enk水平显著升高。给药10分钟后，培养基中未出现Met-enk和CAs的释放，尽管尼古丁确实导致了Met-enk和CAs的显著释放。给药18小时后，使用0至10（-6）M地塞米松之间的不同剂量，牛肾上腺嗜铬细胞中CAs和Met-enk水平呈剂量反应性增加。在培养的人髓外嗜铬细胞瘤细胞中，地塞米松给药24小时后以剂量依赖性方式显著提高了去甲肾上腺素和Met-enk的水平。这些结果表明，地塞米松不是促分泌剂，但可能与Met-enk和CAs的合成有关。
