年龄和持液对野生型和突变型秀丽隐杆线虫滞育幼虫紫外线辐射敏感性的影响。

Effects of age and liquid holding on the UV-radiation sensitivities of wild-type and mutant Caenorhabditis elegans dauer larvae.

作者信息

Hartman P S

出版信息

Mutat Res. 1984 Sep-Oct;132(3-4):95-9. doi: 10.1016/0167-8817(84)90003-8.

Abstract

The dauer larva is a facultative developmental stage in the life cycle of the nematode Caenorhabditis elegans. Dauer larvae, which can survive under starvation for over 60 days, resume normal development when feeding is resumed. Wild-type (N2) and 4 radiation-sensitive (rad) mutant dauer larvae were tested for their abilities to develop into adults after UV-irradiation. The rad-3 mutant was over 30 times as sensitive as N2; rad-1, rad-2 and rad-7 mutants were not hypersensitive. Irradiation also delayed development in survivors. Wild-type dauer larvae did not differ in radiation sensitivity from 0 through 50 days of age. There was no liquid holding recovery (LHR); that is, survival did not increase when wild-type dauer larvae were held in buffer after irradiation.

摘要

dauer幼虫是秀丽隐杆线虫生命周期中的一个兼性发育阶段。dauer幼虫能够在饥饿状态下存活60多天，恢复进食后会恢复正常发育。对野生型（N2）和4种辐射敏感（rad）突变体dauer幼虫进行了紫外线照射后发育成成虫能力的测试。rad-3突变体的敏感性是N2的30多倍；rad-1、rad-2和rad-7突变体没有超敏反应。辐射也延迟了幸存者的发育。野生型dauer幼虫在0至50日龄期间的辐射敏感性没有差异。没有液体保持恢复（LHR）；也就是说，野生型dauer幼虫在照射后置于缓冲液中时存活率没有增加。