Cameron C M, Bester F J, Du Toit D J
Onderstepoort J Vet Res. 1984 Jun;51(2):97-102.
An appreciable level of immunity from intraperitoneal infection with Pasteurella haemolytica was established in mice by using a vaccine prepared in a conventional bacteriological culture medium, with aluminium hydroxide gel as adjuvant. The level of immunity could not be elevated by using bacteria grown in tissue culture media, enriched brain heart infusion broth, the addition of serum to the media or by using bacteria that had been harvested in the logarithmic growth phase. Although various extracts of the bacteria elicited a distinct immunity, the immunogenicity of vaccines containing bacteria could not be enhanced by augmentation with those products. The potential application of the vaccine in cattle and sheep is discussed.
通过使用在常规细菌培养基中制备的疫苗，并以氢氧化铝凝胶作为佐剂，在小鼠中建立了对溶血巴斯德氏菌腹腔感染的显著免疫水平。使用在组织培养基、富集脑心浸液肉汤中生长的细菌、向培养基中添加血清或使用处于对数生长期收获的细菌，免疫水平均无法提高。尽管细菌的各种提取物引发了明显的免疫反应，但含有细菌的疫苗的免疫原性不能通过添加这些产品来增强。讨论了该疫苗在牛和羊中的潜在应用。
