Layton H W
Avian Dis. 1984 Oct-Dec;28(4):1086-95.
Pasteurella multocida (PM) isolates produced dense growth in tryptic soy broth and modified tryptose broth (MTB) when the media were continuously shaken or aerated. In carboys containing 15 liters of aerated MTB, the growth exceeded an absorbance of 0.9 and contained about 10(10) colony-forming units per ml. Bacterins prepared from PM isolates grown in MTB were injected subcutaneously into ducklings at 2 and 3 weeks of age. Such ducklings experienced significantly less mortality than unimmunized controls following homologous challenge at 4, 5, or 6 weeks of age. Similar protection was provided against challenge by a heterologous isolate (same serotype). Six-week-old ducklings given a single injection of an oil-emulsified PM bacterin developed immunity that lasted for 8 weeks.
多杀巴斯德氏菌（PM）分离株在胰蛋白胨大豆肉汤和改良胰蛋白胨肉汤（MTB）中连续振荡或通气培养时生长旺盛。在装有15升通气MTB的大玻璃瓶中培养，其生长吸光度超过0.9，每毫升约含10¹⁰个菌落形成单位。用在MTB中培养的PM分离株制备的菌苗，在雏鸭2周龄和3周龄时皮下注射。在4周龄、5周龄或6周龄进行同源攻击后，这些雏鸭的死亡率明显低于未免疫的对照雏鸭。针对异源分离株（相同血清型）的攻击也提供了类似的保护。单次注射油乳化PM菌苗的6周龄雏鸭产生的免疫力可持续8周。
