Harrison L J, Parkhouse R M, Sewell M M
Parasitology. 1984 Aug;88 ( Pt 4):659-63. doi: 10.1017/s0031182000085577.
The antibody response of the appropriate hosts (cattle) to Taenia saginata larvae was compared with that of an inappropriate host (Balb/c mice) using gel electrophoresis followed by immunoelectrotransfer blot techniques (Western blotting). Three groups of cattle were included, those known to be resistant to challenge infection because of repeated oral challenge with T. saginata eggs over a 1-year period, a previously infected but known susceptible group and a group of uninfected controls. Serum from the mice and the two groups of infected cattle contained antibodies recognizing different 'target' antigens, some of which may be related to host resistance. The potential value of the technique of Western blotting in dissecting the humoral response of a particular host species to parasitic infection and in the identification of those antigens suitable for the production of effective vaccines is discussed.
运用凝胶电泳和免疫电转移印迹技术（蛋白质免疫印迹法），比较了合适宿主（牛）和不合适宿主（Balb/c小鼠）对牛带绦虫幼虫的抗体反应。研究纳入了三组牛，一组是在过去1年中因反复经口感染牛带绦虫卵而对攻击感染具有抗性的牛，一组是先前已感染但已知为易感的牛，还有一组是未感染的对照牛。小鼠以及两组感染牛的血清中含有能识别不同“靶”抗原的抗体，其中一些抗原可能与宿主抗性有关。本文讨论了蛋白质免疫印迹技术在剖析特定宿主物种对寄生虫感染的体液反应以及鉴定适合用于生产有效疫苗的抗原方面的潜在价值。
