Jeon Hyeong-Kyu, Eom Keeseon S
Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine, Cheongju, Korea.
Korean J Parasitol. 2009 Mar;47(1):73-7. doi: 10.3347/kjp.2009.47.1.73. Epub 2009 Mar 12.
Differential diagnosis of Taenia asiatica infection from other human taeniases by serology has been tested. An enzyme-linked immunoelectrotransfer blot (EITB) was applied to subjected human sera and tapeworm materials. Thirty-eight proteins reactive to serum IgG were observed between 121 and 10 kDa in adult worms, and more than 22 serum-reactive components between 97 kDa and 21.5 kDa were observed in eggs of T. asiatica. Antigens of adult T. asiatica revealed immunoblot bands between 120 and 21.5 kDa against T. asiatica infected sera. Antigens of adult Taenia saginata revealed 110-100, 66, 58-56, and 46 kDa immunoblot bands against T. asiatica infected sera. Antigens of adult Taenia solium also revealed 99-97, 68-66, and 46 kDa bands against T. asiatica infected sera. The immunoblot band of 21.5 kDa exhibited specificity to T. asiatica.
通过血清学对亚洲带绦虫感染与其他人体绦虫病进行鉴别诊断已得到验证。将酶联免疫电转印印迹法（EITB）应用于人体血清和绦虫材料。在成虫中观察到38种与血清IgG反应的蛋白质，分子量在121至10 kDa之间，在亚洲带绦虫虫卵中观察到22种以上分子量在97 kDa至21.5 kDa之间的血清反应成分。亚洲带绦虫成虫抗原对亚洲带绦虫感染血清显示出120至21.5 kDa之间的免疫印迹条带。牛带绦虫成虫抗原对亚洲带绦虫感染血清显示出110 - 100、66、58 - 56和46 kDa的免疫印迹条带。猪带绦虫成虫抗原对亚洲带绦虫感染血清也显示出99 - 97、68 - 66和46 kDa的条带。21.5 kDa的免疫印迹条带对亚洲带绦虫具有特异性。
