圆二色性测量表明，在寡脱氧核苷酸双螺旋的反平行链之间，C.C⁺碱基对可以与A.T碱基对共存。
Circular dichroism measurements show that C.C+ base pairs can coexist with A.T base pairs between antiparallel strands of an oligodeoxynucleotide double-helix.
作者信息
Gray D M, Cui T, Ratliff R L
出版信息
Nucleic Acids Res. 1984 Oct 11;12(19):7565-80. doi: 10.1093/nar/12.19.7565.
Abstract
We have studied the coil-to-helix transition of the DNA oligomer d(C4A4T4C4), using circular dichroism measurements to monitor the formation of A.T base pairs within the central self-complementary A4T4 region and the formation of protonated C.C+ base pairs at the ends of the oligomer. We found that both A.T and C.C+ base pairs formed in a coordinated fashion as the temperature and pH were lowered. The CD data of the helix form of the oligomer were consistent with the presence of paired oligomers, but not with hairpin loops. The pKa for formation of C.C+ base pairs between the C4 ends of the oligomer was higher than the pKa for formation of C.C+ base pairs in d(C8), indicating that the formation of C.C+ base pairs in the oligomer was influenced by the presence of a paired A4T4 region. We conclude that A.T and C.C+ base pairs coexist in the self-complex of the oligomer and, therefore, that C.C+ base pairs can form between antiparallel DNA strands.
摘要
我们利用圆二色性测量方法，研究了DNA寡聚物d(C4A4T4C4)从线圈结构到螺旋结构的转变过程，以监测中央自互补A4T4区域内A.T碱基对的形成以及寡聚物末端质子化C.C+碱基对的形成。我们发现，随着温度和pH值的降低，A.T和C.C+碱基对以协同方式形成。该寡聚物螺旋形式的圆二色性数据与配对寡聚物的存在一致，但与发夹环不一致。该寡聚物C4末端之间形成C.C+碱基对的pKa高于d(C8)中形成C.C+碱基对的pKa，这表明该寡聚物中C.C+碱基对的形成受到配对A4T4区域存在的影响。我们得出结论，A.T和C.C+碱基对共存于该寡聚物的自复合体中，因此，C.C+碱基对可以在反平行DNA链之间形成。
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