Nunez A A, Tan D T
Physiol Behav. 1984 May;32(5):717-21. doi: 10.1016/0031-9384(84)90184-7.
The effects of testosterone and its metabolites (estradiol and 5 alpha-dihydrotestosterone) on male mouse ultrasonic vocalizations were investigated using Swiss-Webster mice. In the first experiment daily treatment with 50 micrograms or 200 micrograms of testosterone propionate (TP) restored ultrasonic calling in castrated sexually naive males. Treatment with 200 micrograms of dihydrotestosterone propionate (DHTP) also activated ultrasound production, but treatment with 50 micrograms of DHTP or estradiol benzoate (EB 0.5 microgram and 2 micrograms/day) failed to have a significant effect on this measure. In experiment two, the animals used had shown male sexual behavior before castration. These sexually experienced males showed a marked reduction in ultrasound production after castration but significantly increased ultrasound production after daily treatment with either TP (50 micrograms or 200 micrograms), DHTP (50 micrograms or 200 micrograms) or 2 micrograms of EB. Hormones that facilitated ultrasound calling also activated other components of male sexual behavior in this and previous studies with Swiss-Webster mice. The results indicate that hormone dose and precastration sexual experience interact to determine the effects of postcastration treatment on male vocalizations.
利用瑞士韦伯斯特小鼠研究了睾酮及其代谢产物（雌二醇和5α - 双氢睾酮）对雄性小鼠超声波发声的影响。在第一个实验中，每天用50微克或200微克丙酸睾酮（TP）对阉割后的性幼稚雄性小鼠进行处理，可恢复其超声波鸣叫。用200微克丙酸双氢睾酮（DHTP）处理也能激活超声波发声，但用50微克DHTP或苯甲酸雌二醇（EB，0.5微克/天和2微克/天）处理对此指标没有显著影响。在第二个实验中，所使用的动物在阉割前已表现出雄性性行为。这些有性经验的雄性小鼠在阉割后超声波发声明显减少，但在用TP（50微克或200微克）、DHTP（50微克或200微克）或2微克EB每天处理后，超声波发声显著增加。在本研究以及之前对瑞士韦伯斯特小鼠的研究中，促进超声波鸣叫的激素也激活了雄性性行为的其他成分。结果表明，激素剂量和阉割前的性经验相互作用，决定了阉割后处理对雄性发声的影响。
