Gutierrez O, Salinas R
Physiol Behav. 1984 Jun;32(6):1033-5. doi: 10.1016/0031-9384(84)90298-1.
Consistent penetration of cell membranes by micropipettes is facilitated by using electrode accelerators or high velocity step drives. Notwithstanding, much intracellular work is still done with conventional mechanical or hydraulic drives; cell membrane penetration is achieved by means of gentle taps on any convenient part of the set up. A remote control device is described which performs this function and is compact enough to be fixed on either the microelectrode holder or the preparation mounting. It consists of a small magnetized rod freely suspended in a pot-core coil. A current pulse through the coil jolts the rod; the inertial reaction of the coil frame provides the sudden movement required by the micropipette tip to overcome the elastic resistance of the cell membrane.
使用电极加速器或高速步进驱动器有助于微吸管持续穿透细胞膜。尽管如此，许多细胞内操作仍使用传统的机械或液压驱动器进行；通过轻轻敲击装置的任何方便部位来实现细胞膜穿透。本文描述了一种遥控装置，它具有上述功能，且结构紧凑，足以固定在微电极支架或标本固定架上。它由一根自由悬挂在罐形磁芯线圈中的小磁化棒组成。通过线圈的电流脉冲会使棒震动；线圈框架的惯性反作用力为微吸管尖端提供克服细胞膜弹性阻力所需的突然运动。
