Flaming D G, Brown K T
J Neurosci Methods. 1982 Jul;6(1-2):91-102. doi: 10.1016/0165-0270(82)90019-x.
Though the ultrafine short tips provided by our micropipette puller are helpful for many preparations, longer tips are still needed for some cases where target cells for intracellular work are deeply embedded in tissue. Because the original 'loop' type of heating filament proved unsuitable for widening to provide the longer tips, we studied simpler forms of heating filaments. By comparison with the common loop design, a rectangular trough filament proved to have many advantages without any significant disadvantage. In particular, this type of filament may be used at varying widths to provide long tips as well as short ones. Thus our micropipette puller can now provide tip lengths that extend upward by continuous gradations from about 6 to 27 mm, while maintaining tip diameter in the ultrafine range of 0.1 micrometer or less. These long tips have proved more reliable and free from bending than long tips traditionally provided by the Livingston puller. By using narrow filaments in conjunction with other parameters that influence tip size, tips have also formed with diameters up to 1.5 micrometers and lengths of only 2-3 mm.
尽管我们的微量移液器拉制器提供的超细短吸头对许多制备工作很有帮助，但在某些情况下，细胞内操作的靶细胞深深嵌入组织中时，仍需要更长的吸头。由于原来的“环形”加热丝被证明不适合加宽以提供更长的吸头，我们研究了更简单形式的加热丝。与普通的环形设计相比，矩形槽加热丝被证明有许多优点且没有任何明显缺点。特别是，这种类型的加热丝可以以不同宽度使用，以提供长吸头和短吸头。因此，我们的微量移液器拉制器现在可以提供向上连续渐变的吸头长度，从约6毫米到27毫米，同时将吸头直径保持在0.1微米或更小的超细范围内。这些长吸头已被证明比利文斯顿拉制器传统提供的长吸头更可靠且不易弯曲。通过结合使用窄加热丝和其他影响吸头尺寸的参数，也形成了直径达1.5微米且长度仅为2 - 3毫米的吸头。
