Prpić-Majić D, Telisman S, Kezić S
Scand J Work Environ Health. 1984 Aug;10(4):235-8. doi: 10.5271/sjweh.2336.
The effect of lead (Pb) and ethanol (EtOH) interaction on the inhibition of erythrocyte delta-aminolevulinic acid dehydratase (ALAD) was investigated in human blood in vitro. Two different doses of ethanol (equivalent to 16.28 mmol of EtOH/l of blood and 108.53 mmol of EtOH/l of blood) and lead (equivalent to 2.17 mumol of Pb/l of blood and 4.34 mumol of Pb/l of blood) were examined separately and in combination. The dose-effect (EtOH-ALAD) relationship for a wide range of ethanol concentrations (0-217.06 mmol of EtOH/l of blood) was also investigated. The results obtained indicate that ethanol by itself does not inhibit ALAD, while lead does it readily. Neither ethanol concentrations significantly altered ALAD activity. The dose-effect (EtOH-ALAD) relationship did not reveal any inhibitory effect of ethanol on ALAD either; however, a weak trend towards increased ALAD activity was found. The effect of ethanol combined with lead indicated no significant difference as compared to the effect of the same dose of lead per se; however, a weak trend towards decreased ALAD activity was found. These findings support the hypothesis that the effect of ethanol on the transient inhibition of ALAD activity in vivo does not occur directly, but possibly through the intermediary action of lead from the body lead pool.
在体外人体血液中研究了铅（Pb）与乙醇（EtOH）相互作用对红细胞δ-氨基-γ-酮戊酸脱水酶（ALAD）抑制作用的影响。分别检测了两种不同剂量的乙醇（相当于每升血液中含16.28 mmol乙醇和108.53 mmol乙醇）和铅（相当于每升血液中含2.17 μmol铅和4.34 μmol铅），以及它们联合作用的情况。还研究了广泛乙醇浓度范围（0 - 217.06 mmol乙醇/升血液）下的剂量效应（乙醇-ALAD）关系。所得结果表明，乙醇本身并不抑制ALAD，而铅则很容易抑制。乙醇浓度均未显著改变ALAD活性。剂量效应（乙醇-ALAD）关系也未显示乙醇对ALAD有任何抑制作用；然而，发现有ALAD活性增加的微弱趋势。乙醇与铅联合作用的效果与相同剂量铅单独作用的效果相比无显著差异；不过，发现有ALAD活性降低的微弱趋势。这些发现支持了以下假设：乙醇对体内ALAD活性短暂抑制的作用不是直接发生的，而是可能通过体内铅库中的铅的中介作用。
