Galli S J, Hammel I
Science. 1984 Nov 9;226(4675):710-3. doi: 10.1126/science.6494907.
It has been suggested that reserpine blocks expression of delayed hypersensitivity in mice because it depletes stores of the vasoactive amine serotonin in mast cells. To determine whether mast cell serotonin or other mast cell-derived mediators are essential for delayed hypersensitivity, responses to contact sensitizers in mast cell-deficient W/Wv or Sl/Sld mice were studied. Because blood platelets represent another potential source of serotonin in delayed hypersensitivity responses, beige mice, whose platelets contain less than 1 percent of the normal levels of serotonin, were also examined. By the criteria of tissue swelling, infiltration of iodinated leukocytes, or histology, mast cell-deficient or beige mice expressed delayed hypersensitivity reactions whose intensity generally equaled or exceeded that of reactions in littermate controls. In addition, reserpine blocked delayed hypersensitivity in W/Wv and beige mice, suggesting that effects on mast cell or platelet serotonin cannot explain this drug's action in delayed hypersensitivity.
有人提出，利血平会阻断小鼠迟发型超敏反应的表达，因为它会耗尽肥大细胞中血管活性胺血清素的储存。为了确定肥大细胞血清素或其他肥大细胞衍生介质对迟发型超敏反应是否至关重要，研究了肥大细胞缺陷的W/Wv或Sl/Sld小鼠对接触性致敏剂的反应。由于血小板是迟发型超敏反应中血清素的另一个潜在来源，还检查了米色小鼠，其血小板中血清素含量低于正常水平的1%。根据组织肿胀、碘化白细胞浸润或组织学标准，肥大细胞缺陷或米色小鼠表现出迟发型超敏反应，其强度通常等于或超过同窝对照的反应。此外，利血平阻断了W/Wv和米色小鼠的迟发型超敏反应，这表明对肥大细胞或血小板血清素的影响无法解释该药物在迟发型超敏反应中的作用。
