Sakariassen K S, Banga J D, de Groot P G, Sixma J J
Thromb Haemost. 1984 Aug 31;52(1):60-5.
Platelet interaction with subendothelium of human renal and umbilical arteries and with the extracellular matrix produced by cultured human venous endothelial cells was compared in flowing citrated blood by using an annular and a rectangular perfusion chamber. The renal arteries were post mortem specimens from adults showing, often pronounced, intimal fibrosis, whereas the umbilical arteries had well organized parallel arranged smooth muscle cells, without elastic membranes. The extracellular matrix obtained after removal of endothelial cells with Triton X-100 was homogenously attached to its substratum. Significantly more platelets adhered to the extracellular matrix than to the subendothelia. This discrepancy was most pronounced in reconstituted blood with plasma from a patient with homozygous severe von Willebrand's disease (subtype III). No differences in platelet adherence and platelet aggregate formation were noted between the subendothelia. Platelet aggregate formation was poor on all surfaces. These data indicate that the extracellular matrix produced by endothelial cells is at least as reactive for the interaction with platelets as subendothelium, probably partly synthesized by smooth muscle cells.
使用环形和矩形灌注室，在流动的枸橼酸盐血液中比较了血小板与人肾动脉和脐动脉的内皮下层以及与培养的人静脉内皮细胞产生的细胞外基质的相互作用。肾动脉是来自成人的尸检标本，常显示明显的内膜纤维化，而脐动脉有排列整齐的平行平滑肌细胞，无弹性膜。用Triton X-100去除内皮细胞后获得的细胞外基质均匀地附着于其基质上。与内皮下层相比，有更多的血小板黏附于细胞外基质。这种差异在患有纯合子严重血管性血友病（III型）患者的血浆重构血液中最为明显。在内皮下层之间，未观察到血小板黏附和血小板聚集体形成的差异。在所有表面上，血小板聚集体形成均较差。这些数据表明，内皮细胞产生的细胞外基质与内皮下层至少对与血小板的相互作用一样具有反应性，可能部分由平滑肌细胞合成。
