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甲型H1N1流感病毒经超声处理后在光散射、吸收、荧光及生物学特性方面的变化

Changes in light scattering, absorption, fluorescence and biological characteristics of influenza virus A(H1N1) exposed to sonication.

作者信息

Zaharia C N, Petrescu A

出版信息

Virologie. 1984 Jul-Sep;35(3):193-206.

PMID:6495648
Abstract

Influenza virus A/Brasil 11/78 (H1N1) was sonicated for different time intervals (2-328 sec) in a continuous flow Ultrasonics F600 device at 20 kHz, 600 W. Marked changes in the structure of the virions were made evident by light scattering, absorption and fluorescence measurements. Light scattering data indicated that the turbidity of the influenza virus samples is inversely proportional to the square of the wavelength. A sharp decrease in hemagglutinating activity and infectivity occurred following sonication for more than 50 sec.

摘要

将甲型/巴西11/78(H1N1)流感病毒在连续流动的Ultrasonics F600装置中于20千赫、600瓦功率下超声处理不同时间间隔(2 - 328秒)。通过光散射、吸收和荧光测量发现病毒粒子结构发生了显著变化。光散射数据表明,流感病毒样本的浊度与波长的平方成反比。超声处理超过50秒后,血凝活性和感染力急剧下降。

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