一种用于检测石房蛤毒素和河豚毒素的竞争性置换测定法。
A competitive displacement assay to detect saxitoxin and tetrodotoxin.
作者信息
Davio S R, Fontelo P A
出版信息
Anal Biochem. 1984 Aug 15;141(1):199-204. doi: 10.1016/0003-2697(84)90446-9.
An assay is described which detects saxitoxin (STX) and tetrodotoxin (TTX) by their competitive displacement of [3H]saxitoxin from its receptor in rat brain membranes. The assay has a sensitivity of 0.15 ng STX/ml and 0.8 ng TTX/ml for buffer samples. The assay was also applied to detection of these toxins in unextracted human plasma and found to have a sensitivity of 0.5 ng STX/ml and 0.6 ng TTX/ml. The competitive displacement assay appears to be the most sensitive procedure yet for detection of STX and TTX.
本文描述了一种检测方法，该方法通过检测河鲀毒素（STX）和河豚毒素（TTX）在大鼠脑膜中竞争性取代[3H]河鲀毒素与其受体的结合，来测定这两种毒素。该检测方法对缓冲液样本中STX的检测灵敏度为0.15 ng/ml，对TTX的检测灵敏度为0.8 ng/ml。该检测方法还用于检测未经提取的人血浆中的这些毒素，发现其对STX的检测灵敏度为0.5 ng/ml，对TTX的检测灵敏度为0.6 ng/ml。竞争性取代检测法似乎是目前检测STX和TTX最灵敏的方法。
Zotero 插件