一种用于检测河豚毒素、石房蛤毒素及相关毒素的组织培养测定法。
A tissue culture assay for tetrodotoxin, saxitoxin and related toxins.
作者信息
Kogure K, Tamplin M L, Simidu U, Colwell R R
机构信息
Center of Marine Biotechnology, University of Maryland, Baltimore 21202.
出版信息
Toxicon. 1988;26(2):191-7. doi: 10.1016/0041-0101(88)90171-7.
In the presence of ouabain, veratridine enhances sodium influx in the mouse neuroblastoma cell line Neuro-2A (ATCC, CCL131), causing cellular swelling and subsequent death. Tetrodotoxin (puffer fish toxin) or saxitoxin (paralytic shellfish poison), both of which block the sodium channel of excitable membranes, antagonize this effect, enabling cell growth to continue. This phenomenon was used as the basis of a new assay for these toxins. It is also possible to estimate the quantity of TTX from the relationship between TTX concentration and percentage of living cells. This new method is simple, inexpensive, and sensitive, and may replace the conventional mouse bioassay.
在哇巴因存在的情况下，藜芦定可增强小鼠神经母细胞瘤细胞系Neuro-2A（美国典型培养物保藏中心，CCL131）中的钠内流，导致细胞肿胀并随后死亡。河豚毒素（来自河豚的毒素）或石房蛤毒素（麻痹性贝类毒素）均可阻断可兴奋膜的钠通道，二者均能拮抗这种效应，使细胞生长得以继续。这一现象被用作这些毒素新检测方法的基础。还可以根据河豚毒素浓度与活细胞百分比之间的关系来估算河豚毒素的量。这种新方法简单、廉价且灵敏，可能会取代传统的小鼠生物测定法。
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