Bookjans G, Stummann B M, Henningsen K W
Anal Biochem. 1984 Aug 15;141(1):244-7. doi: 10.1016/0003-2697(84)90452-4.
A simple, rapid, and inexpensive method for the preparation and purification of chloroplast DNA (cpDNA) from pea has been developed. The crucial step is the isolation of chloroplasts in a medium of high ionic strength (I congruent equal to 1.40 M). CpDNA from pea prepared according to this method has successfully been used for restriction enzyme mapping, Southern transfers, and cloning.
已开发出一种从豌豆中制备和纯化叶绿体DNA（cpDNA）的简单、快速且廉价的方法。关键步骤是在高离子强度（I等于1.40 M）的介质中分离叶绿体。按照此方法制备的豌豆cpDNA已成功用于限制性酶切图谱分析、Southern杂交和克隆。
