亚硫酸钠作为牛胰核糖核酸酶A酸水解中的抗氧化剂。

Sodium sulfite as an antioxidant in the acid hydrolysis of bovine pancreatic ribonuclease A.

作者信息

Swadesh J K, Thannhauser T W, Scheraga H A

出版信息

Anal Biochem. 1984 Sep;141(2):397-401. doi: 10.1016/0003-2697(84)90060-5.

Abstract

Treatment of hydrochloric acid with sodium sulfite prior to the acid hydrolysis of bovine pancreatic ribonuclease A has been found to suppress the oxidation of cystine, methionine, and tyrosine without adversely affecting the recoveries of other amino acids. Statistical analysis of the results indicated that the assumption of the independence of the mean and the variance, an assumption commonly used in the evaluation of the effects of various treatments, may not be valid in evaluating antioxidants used in the acid hydrolysis of proteins.

摘要

在对牛胰核糖核酸酶A进行酸水解之前，先用亚硫酸钠处理盐酸，已发现这样做可以抑制胱氨酸、蛋氨酸和酪氨酸的氧化，而不会对其他氨基酸的回收率产生不利影响。对结果的统计分析表明，均值和方差独立性的假设（这是评估各种处理效果时常用的假设）在评估用于蛋白质酸水解的抗氧化剂时可能无效。