微量技术用于小血样中巴比妥类药物的气相色谱测定。

Microtechniques for the gas chromatographic determination of barbiturates in small blood samples.

作者信息

Dünges W, Bergheim-Irps E, Straub H, Kaiser R E

出版信息

J Chromatogr. 1978 Mar 1;145(2):265-74. doi: 10.1016/s0378-4347(00)81347-x.

Abstract

A method for the determination of therapeutic levels of barbituric acids in 25 microliter of whole blood is described. After extraction and controlled concentration of the extract to a volume of 5 microliter, the barbituric acids are N,N'-dimethylated using a microrefluxer. Of the total extract 20-100% is injected into the gas chromatograph. Low blanks, recoveries of 70--80% and peak ratios that are comparable to those in calibration experiments are obtained provided the detailed working instructions are followed strictly. In addition, barbiturates were determined (1 ng in 25 microliter blood) using column-switching devices and nitrogen-sensitive detection.

摘要

本文描述了一种测定25微升全血中巴比妥酸治疗水平的方法。萃取后，将萃取物浓缩至5微升，使用微型回流器对巴比妥酸进行N,N'-二甲基化。将20%-100%的总萃取物注入气相色谱仪。只要严格遵循详细的操作说明，就能获得低空白值、70%-80%的回收率以及与校准实验相当的峰比。此外，使用柱切换装置和氮敏检测法测定了（25微升血液中1纳克）巴比妥酸盐。