Masana M, de Toranzo E G, Castro J A
Arch Int Pharmacodyn Ther. 1984 Jul;270(1):4-10.
Rat liver microsomes exhibit nifurtimox (NFX) nitroreductase activity, which is mostly NADPH-dependent and is completely abolished by heating and under an atmosphere of air. Pure carbon monoxide inhibits for 28% microsomal NFX nitroreductase activity while FAD 1 mM significantly enhances it. Smaller activities than in liver were found in brain, small intestine, testes, lung and heart. Rat liver cytosol also showed NFX nitroreductase activity using either hypoxanthine or N-methylnicotinamide as substrates. These activities were inhibited by allopurinol or menadione respectively. Results suggest that cytochrome P-450, NADPH cytochrome c reductase, xanthinoxidase and aldehyde oxidase are able to reduce NFX nitrogroups in rat liver and other tissues.
大鼠肝脏微粒体具有硝呋替莫（NFX）硝基还原酶活性，该活性大多依赖于NADPH，并且通过加热和在空气氛围下会完全丧失。纯一氧化碳抑制微粒体NFX硝基还原酶活性达28%，而1 mM的黄素腺嘌呤二核苷酸（FAD）则显著增强该活性。在脑、小肠、睾丸、肺和心脏中发现的活性低于肝脏中的活性。大鼠肝脏胞质溶胶以次黄嘌呤或N-甲基烟酰胺作为底物时也表现出NFX硝基还原酶活性。这些活性分别被别嘌呤醇或甲萘醌抑制。结果表明，细胞色素P-450、NADPH细胞色素c还原酶、黄嘌呤氧化酶和醛氧化酶能够还原大鼠肝脏及其他组织中的NFX硝基。
