一种用于分离具有高光合能力的完整叶绿体的改进方法。
An improved procedure for the isolation of intact chloroplasts of high photosynthetic capacity.
作者信息
Cerović Z G, Plesnicar M
出版信息
Biochem J. 1984 Oct 15;223(2):543-5. doi: 10.1042/bj2230543.
Abstract
An improved procedure for the mechanical isolation of chloroplasts of high degree of intactness (90-95%) and photosynthetic capacity (25-50 mmol of O2/s per mol of chlorophyll) is described. The combination of pea plants (Pisum sativum L.) as starting material and the high reproducibility of the procedure readily and cheaply yields reliable intact chloroplasts for photosynthetic studies.
摘要
本文描述了一种改进的方法，用于机械分离高度完整（90-95%）且具有光合能力（每摩尔叶绿素每秒25-50微摩尔氧气）的叶绿体。以豌豆植株（Pisum sativum L.）作为起始材料，结合该方法的高重现性，能够轻松且廉价地获得用于光合研究的可靠完整叶绿体。
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