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Stereospecific incorporation of hydrogens from NADPH in elongation of very long fatty acyl-CoA by swine cerebral microsomes.

作者信息

Yoshida S, Takeshita M, Kawaguchi A

出版信息

Biochem Biophys Res Commun. 1984 Oct 30;124(2):322-8. doi: 10.1016/0006-291x(84)91556-0.

DOI:10.1016/0006-291x(84)91556-0
PMID:6497886
Abstract

The elongation of arachidoyl-CoA by swine cerebral microsomes resulted in the production of behenic acid (22:0) and lignoceric acid (24:0) concomitantly. When 4S-[4-2H1]NADPH was used for the elongation of arachidoyl-CoA, the incorporation of two deuterium atoms into 22:0 was observed by the technique of mass fragmentography. Furthermore, the incorporation of four deuterium atoms into 24:0 was also detected. On the other hand, when 4R-[4-2H1]NADPH was used, no deuterium was incorporated into the elongated products.

摘要

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