Wegrowski J, Robert A M, Moczar M
Biochem Pharmacol. 1984 Nov 1;33(21):3491-7. doi: 10.1016/0006-2952(84)90125-4.
Rabbits were fed with normal (group 1 and 2) and cholesterol rich diets (group 3 and 4) concomitantly to a daily peroral administration of 50 mg/kg procyanidolic oligomers (PCO) to groups 2 and 4. After 10 weeks, the cholesterol content of the blood serum and the excised aortic intima-media were significantly higher in groups 3 and 4 than in groups 1 and 2. The DNA, hydroxyproline, uronic acid contents were similar in aortic dry weight basis in all four groups. The intima-media samples were extracted successively with 0.15 M NaCl, 0.02 M sodium phosphate pH 7.4 (NaCl extract) and with 4 M guanidinium chloride, 0.05 M sodium acetate pH 5.8 prior (G1 extract) and following (G2 extract) hydrolysis of the collagen with collagenase. The cholesterol contents of G1 extracts were higher in groups 2 and 4 than in groups 1 and 3. The cholesterol content of aortic elastin increased with cholesterol feeding (group 3). With simultaneous administration of cholesterol and PCO the cholesterol content of aortic elastin in group 4 was significantly lower than in group 3. The uronic acid contents increased in G1 extracts and in the collagenase digest with PCO treatment of both normal and hypercholesterolemic rabbits. The ratio of dermatan-sulphate to chondroitin-sulphate decreased with hypercholesterolemia (group 3) and with PCO (group 2 and 4). The parallelism between increased cholesterol and uronic acid contents and modified glycosaminoglycan composition in G1 extract, indicate that the interaction of cholesterol with macromolecules of the aorta can be modulated by PCO. This drug modifies the extractibility of aortic cholesterol and glycosaminoglycans and reduces the association of cholesterol to elastin.
给兔子喂食正常饮食（第1组和第2组）和富含胆固醇的饮食（第3组和第4组），同时第2组和第4组每天口服50毫克/千克的原花青素低聚物（PCO）。10周后，第3组和第4组血清和切除的主动脉内膜中层的胆固醇含量显著高于第1组和第2组。所有四组以主动脉干重计的DNA、羟脯氨酸、糖醛酸含量相似。内膜中层样本先用0.15M氯化钠、0.02M磷酸钠pH 7.4（氯化钠提取物）依次提取，然后在用胶原酶水解胶原蛋白之前（G1提取物）和之后（G2提取物）用4M氯化胍、0.05M醋酸钠pH 5.8提取。第2组和第4组G1提取物中的胆固醇含量高于第1组和第3组。随着胆固醇喂养（第3组），主动脉弹性蛋白的胆固醇含量增加。同时给予胆固醇和PCO时，第4组主动脉弹性蛋白的胆固醇含量显著低于第3组。正常和高胆固醇血症兔子经PCO处理后，G1提取物和胶原酶消化物中的糖醛酸含量增加。随着高胆固醇血症（第3组）和PCO（第2组和第4组），硫酸皮肤素与硫酸软骨素的比例降低。G1提取物中胆固醇和糖醛酸含量增加以及糖胺聚糖组成改变之间的平行关系表明，胆固醇与主动脉大分子的相互作用可被PCO调节。这种药物改变了主动脉胆固醇和糖胺聚糖的可提取性，并减少了胆固醇与弹性蛋白的结合。
