Takada M, Yuzuriha T, Katayama K, Iwamoto K, Sunamoto J
Biochim Biophys Acta. 1984 Nov 28;802(2):237-44. doi: 10.1016/0304-4165(84)90167-3.
Liposomes labeled with [14C]coenzyme Q10 in the lipid bilayer were coated with various polysaccharide derivatives, i.e., palmitoyl conjugates of pullulan, pullulan phosphate, amylopectin, amylopectin phosphate and amylopectin sulfate. The kinetics of disposition and the tissue distribution of [14C]coenzyme Q10 after intravenous injection of the liposomes into guinea pigs were investigated. Lung uptake of radioactivity after injection of the O-palmitoyl amylopectin- and O-palmitoyl amylopectin phosphate-coated liposomes was 5- and 3-times higher, respectively, at 30 min after injection than that of the conventional liposomes. For doubly labeled liposomes with [3H]inulin and [14C]coenzyme Q10, the 3H/14C ratios in the lung, spleen and heart were similar to one another. Urinary excretion of [3H]inulin encapsulated in O-palmitoyl amylopectin-coated liposomes was much lower than that of unencapsulated [3H]inulin. These observations suggest that the O-palmitoyl amylopectin-coated liposomes are rather stable in vivo and are taken up into tissues in the intact form.
脂质双层中标记有[14C]辅酶Q10的脂质体用各种多糖衍生物进行包被，即支链淀粉的棕榈酰缀合物、磷酸化支链淀粉、支链淀粉、磷酸化支链淀粉和硫酸化支链淀粉。研究了将脂质体静脉注射到豚鼠体内后，[14C]辅酶Q10的处置动力学和组织分布。注射O-棕榈酰支链淀粉和O-棕榈酰磷酸化支链淀粉包被的脂质体后，在注射后30分钟时，肺部对放射性的摄取分别比传统脂质体高5倍和3倍。对于用[3H]菊粉和[14C]辅酶Q10进行双重标记的脂质体，肺、脾和心脏中的3H/14C比值彼此相似。封装在O-棕榈酰支链淀粉包被的脂质体中的[3H]菊粉的尿排泄量远低于未封装的[3H]菊粉。这些观察结果表明，O-棕榈酰支链淀粉包被的脂质体在体内相当稳定，并以完整形式被摄取到组织中。
