Tomizawa H, Aramaki Y, Fujii Y, Hara T, Suzuki N, Yachi K, Kikuchi H, Tsuchiya S
Tokyo College of Pharmacy, Japan.
Pharm Res. 1993 Apr;10(4):549-52. doi: 10.1023/a:1018945902276.
Uptake of the nonabsorbable marker 6-carboxyfluorescein was investigated both free and encapsulated in liposomes as a function of their surface charge and hydrodynamic diameter in rat Peyer's patch and nonpatch tissue. Significant uptake of the marker occurred only when encapsulated in liposomes consisting of at least 25 mol% phosphatidylserine and was highest in Peyer's patches. 6-Carboxyfluorescein encapsulated in liposomes equal to or greater than 374 nm was preferentially taken up by Peyer's patches. There was a trend to higher uptake in lower intestinal segments. These findings were supported by fluorescence microscopic observations. Uptake by Peyer's patches was specific for negatively charged liposomes as judged from competition studies.
在大鼠派尔集合淋巴结和非派尔集合淋巴结组织中，研究了不可吸收标记物6-羧基荧光素在游离状态以及包封于脂质体中的摄取情况，该摄取情况是其表面电荷和流体动力学直径的函数。仅当标记物包封于至少含有25摩尔%磷脂酰丝氨酸的脂质体中时，才会出现显著摄取，且在派尔集合淋巴结中的摄取量最高。包封于直径等于或大于374 nm脂质体中的6-羧基荧光素优先被派尔集合淋巴结摄取。在小肠下段有摄取量更高的趋势。这些发现得到了荧光显微镜观察结果的支持。从竞争研究判断，派尔集合淋巴结的摄取对带负电荷的脂质体具有特异性。
