Schonfeld A R, Heacock A M, Katzman R
Brain Res. 1984 Nov 12;321(2):377-80. doi: 10.1016/0006-8993(84)90197-5.
Injury to neural tissue elicits the release of neurotrophic substances which support the survival of cultured cholinergic neurons. The aim of the present report was to determine the effect of prior hippocampal injury on the sprouting of mature intrinsic cholinergic septal afferents in vivo. Levels of choline acetyltransferase (CAT) in intrahippocampal iris implants were used as an index of cholinergic regeneration. The results show that prelesioning the hippocampus 1 week prior to insertion of the iris increased CAT levels of implants left for 8 days in situ. Levels of iris CAT were correlated with the trophic content of the surrounding hippocampal tissue. These observations suggest that endogenous trophic factors released as a consequence of neural injury facilitate cholinergic sprouting in vivo.
神经组织损伤会引发神经营养物质的释放，这些物质可支持培养的胆碱能神经元的存活。本报告的目的是确定海马体预先损伤对体内成熟的内在胆碱能隔区传入纤维发芽的影响。海马体内虹膜植入物中胆碱乙酰转移酶（CAT）的水平被用作胆碱能再生的指标。结果显示，在植入虹膜前1周对海马体进行损伤，会使原位留存8天的植入物的CAT水平升高。虹膜CAT水平与周围海马体组织的营养成分相关。这些观察结果表明，神经损伤导致释放的内源性营养因子有助于体内胆碱能纤维的发芽。
