Westphal E, Heidemann H, Müller-Ruchholtz W
Diagn Immunol. 1984;2(2):122-7.
HLA typing is usually performed by directly measuring complement-dependent cytotoxicity on viable peripheral blood lymphocytes as target cells. To overcome the limitations inherent to viable targets, absorption inhibition techniques have been developed. The main drawbacks of most of these techniques are, however, that they are not very feasible and require relatively large amounts of absorbing material and rare antisera. Therefore, we adjusted the multiple microabsorption method (MMA) and tested simultaneously for 16 HLA specificities of the A, B, and C locus on 6 different nonviable tissues. The results of the MMA, when compared with those of the usual microlymphocytotoxicity test (LCT) that was run in parallel, correspond in 96% of the 28 comparable antigen pairs. Only one false negative and no false positive result was found. The absorbing quality of the individual organs differed, as was to be expected: lymph node and spleen rank on the top, followed by liver and kidney, whereas brain and muscle show several negative reactions. Altogether, the MMA proves to be a reliable and practical method for typing nonviable tissues, eg, in hematological diseases or in certain forensic situations.
HLA分型通常通过直接检测以活的外周血淋巴细胞为靶细胞的补体依赖细胞毒性来进行。为克服活靶细胞固有的局限性，已开发出吸收抑制技术。然而，这些技术大多数的主要缺点是不太可行，并且需要相对大量的吸收材料和稀有的抗血清。因此，我们调整了多重微量吸收法（MMA），并在6种不同的非活组织上同时检测A、B和C位点的16种HLA特异性。与同时进行的常规微量淋巴细胞毒性试验（LCT）结果相比，在28对可比抗原对中，MMA结果的符合率为96%。仅发现1例假阴性结果，未发现假阳性结果。正如预期的那样，各个器官的吸收质量有所不同：淋巴结和脾脏位居榜首，其次是肝脏和肾脏，而大脑和肌肉则出现一些阴性反应。总体而言，MMA被证明是一种可靠且实用的非活组织分型方法，例如在血液系统疾病或某些法医情况下。
