Whitsett C F, Priest J H, Priest R E, Marion J
Am J Clin Pathol. 1983 Feb;79(2):186-94. doi: 10.1093/ajcp/79.2.186.
HLA typing was performed on 18 cultures of human amniotic fluid cells using cytotoxicity and absorption technics. Confirmation of antigen assignments was obtained in nine of ten instances, where HLA typing also was performed on cord blood. Three major problems were encountered in performing these studies: (1) complement cytotoxicity, (2) false-positive reactions, and (3) false-negative reactions. False-positive and false-negative reactions occurred more frequently with sera defining HLA-B locus specificities than with sera defining HLA-A locus specificities. Absorption studies were helpful in making antigen assignments when false reactions occurred. Preliminary studies suggest that the frequency of false-positive reactions can be decreased by absorbing HLA typing sera with antigen-negative amniotic fluid cultured cells, buffy coat, or platelets. Accurate antigen assignment is difficult when parental HLA types are unavailable.
采用细胞毒性和吸收技术对18份人羊水细胞培养物进行了HLA分型。在十例中有九例通过对脐血进行HLA分型，确认了抗原分配。进行这些研究时遇到了三个主要问题：（1）补体细胞毒性，（2）假阳性反应，以及（3）假阴性反应。与定义HLA - A位点特异性的血清相比，定义HLA - B位点特异性的血清出现假阳性和假阴性反应的频率更高。当出现假反应时，吸收研究有助于进行抗原分配。初步研究表明，用抗原阴性的羊水培养细胞、血沉棕黄层或血小板吸收HLA分型血清可降低假阳性反应的频率。当父母的HLA类型不可用时，准确的抗原分配很困难。
