Evans D L, Graves S S, Blazer V S, Dawe D L, Gratzek J B
Comp Immunol Microbiol Infect Dis. 1984;7(2):91-100. doi: 10.1016/0147-9571(84)90003-1.
Nonspecific cytotoxic cells (NCC) from fish (Ictalurus punctatus) were treated with different concentrations of retinolacetate and poly I:C. Both in vitro and in vivo experiments were conducted. Retinolacetate significantly increased NCC activity against chromium-51 labeled human B-cell lymphoma target cells (NC-37). Preincubation (treatment prior to adding the labeled target cells) of NCC for 4 to 8 h in 10(-3) to 10(-12) M concentration of retinolacetate produced significant increases in NCC activity compared to treatment during the killing assay only. Similar experiments with different concentrations of poly I:C had no NCC augmenting effects when tested by adding poly I:C either during preincubation periods or during the cytotoxicity assay. Retinolacetate probably produces positive modulation of cytotoxicity by increasing the killing effectiveness of individual NCC, rather than recruiting larger numbers of cytolytic cells. In vitro studies were also conducted by injecting catfish (i.p.) with 1 X, 3X and 5X the daily recommended vitamin A dosages and determining NCC activity after 24, 48 and 72 h treatment. The 1X dose significantly increased NCC activity at 72 h. This increase was not transient because NCC activity after 33-37 days' treatment was significantly higher than controls in the 1X, 2X and 3X groups. Intraperitoneal injections of fish with poly I:C produced no significant increases in NCC activity at 24 or 72 h post-inoculation.
用不同浓度的视黄醇醋酸酯和聚肌胞苷酸（poly I:C）处理斑点叉尾鮰的非特异性细胞毒性细胞（NCC）。进行了体外和体内实验。视黄醇醋酸酯显著提高了NCC对铬-51标记的人B细胞淋巴瘤靶细胞（NC-37）的活性。与仅在杀伤试验期间进行处理相比，将NCC在10⁻³至10⁻¹²M浓度的视黄醇醋酸酯中预孵育（在添加标记靶细胞之前进行处理）4至8小时，可使NCC活性显著增加。当在预孵育期或细胞毒性试验期间添加不同浓度的聚肌胞苷酸进行类似实验时，未观察到对NCC的增强作用。视黄醇醋酸酯可能通过提高单个NCC的杀伤效力而非募集更多的溶细胞性细胞来产生细胞毒性的正向调节。还通过腹腔注射鲶鱼每日推荐维生素A剂量的1倍、3倍和5倍，并在处理24、48和72小时后测定NCC活性进行了体外研究。1倍剂量在72小时时显著增加了NCC活性。这种增加不是短暂的，因为在1倍、2倍和3倍组中，处理33 - 37天后的NCC活性显著高于对照组。腹腔注射聚肌胞苷酸的鱼在接种后24或72小时，NCC活性没有显著增加。
