Maiolini R, Ferrua B, Quaranta J F, Pinoteau A, Euller L, Ziegler G, Massaeyeff R
J Immunol Methods. 1978;20:25-34. doi: 10.1016/0022-1759(78)90241-7.
A sandwich enzyme-immunoassay (EIA) has been applied to the determination of the rheumatoid factor (RF). This non-competeitive assay comprises 3 steps: 1) the RF to be assayed is extracted for the biological medium by an immunosorbent of aggregated IgG linked to cellulose; 2) the solid phase is then incubated with the enzyme-labeled aggregated IgG; 3) the enzymatic activity of the immunosorbent is then measured with a suitable chromogenic reagent. This activity is a direct function of the amount of RF to be assayed. This assay gave reproducible results in the range 0.5-50.0 IU/ml. A good agreement was obtained between the EIA and the Waaler-Rose test but no correlation was obtained with the latex slide-test. This assay permits a quantitation of RF with a good reproducibility (coefficient of variation in the range of 10% for moderately elevated values) and thus allows a closer follow-up of patients. The results do not depend on the interpretation of the technician performing the test, which can be easily automated. Finally, it may detect some RF devoid of agglutinating activity.
一种夹心酶免疫测定法（EIA）已应用于类风湿因子（RF）的测定。这种非竞争性测定法包括3个步骤：1）通过与纤维素相连的聚集IgG免疫吸附剂从生物介质中提取待测定的RF；2）然后将固相与酶标记的聚集IgG一起孵育；3）然后用合适的显色试剂测量免疫吸附剂的酶活性。这种活性是待测定RF量的直接函数。该测定法在0.5 - 50.0 IU/ml范围内给出了可重复的结果。EIA与瓦勒 - 罗斯试验之间取得了良好的一致性，但与乳胶玻片试验未获得相关性。该测定法允许对RF进行定量，具有良好的重现性（对于中度升高的值，变异系数在10%范围内），因此可以更密切地跟踪患者。结果不依赖于进行测试的技术人员的解释，并且可以很容易地实现自动化。最后，它可能检测到一些没有凝集活性的RF。
