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Enzyme-linked immunosorbent assay (ELISA) screening test for detection of rheumatoid factor.

作者信息

Jonsson T, Arnason J A, Valdimarsson H

出版信息

Rheumatol Int. 1986;6(5):199-204. doi: 10.1007/BF00541367.

DOI:10.1007/BF00541367
PMID:3797955
Abstract

A new enzyme-linked immunosorbent assay (ELISA) screening test for total rheumatoid factor (RF) activity is described. Rabbit IgG was used as antigen and enzyme-conjugated monoclonal anti-kappa antibody as third layer. Of 183 samples measured for RF isotype levels, 60 were found to have one or more raised. In terms of raised isotypes the ELISA screening test had a sensitivity of 97% (58/60) while the Rheumaton had a sensitivity of only 75% (45/60). Nearly all discordant false-negative samples had only one RF isotype raised. The ELISA test gave 29% (53/183) and the Rheumaton 34% (63/183) false-positive results. Thus the ELISA test was more specific and sensitive for the detection of raised single RF isotypes than the Rheumaton and Rose-Waaler tests. Moreover, approximately 30% of RA patients were seronegative according to the conventional RF tests but only 8% in the new ELISA system.

摘要

相似文献

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2
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本文引用的文献

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A sensitive radioimmunoassay for quantitation of IgM rheumatoid factor.一种用于定量检测 IgM 类风湿因子的灵敏放射免疫测定法。
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与类风湿因子亚型相比，抗环瓜氨酸肽抗体在类风湿关节炎中的诊断及临床价值
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Population study of the importance of rheumatoid factor isotypes in adults.类风湿因子同种型在成人中的重要性的人群研究。
Ann Rheum Dis. 1992 Jul;51(7):863-8. doi: 10.1136/ard.51.7.863.
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False positive results in class-specific rheumatoid factor (RF) assays due to interaction between RF and Fc fragments of anti-immunoglobulin indicator reagents.由于类风湿因子（RF）与抗免疫球蛋白指示试剂的Fc片段之间相互作用，导致特异性类风湿因子（RF）检测出现假阳性结果。
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Prospective study of early rheumatoid arthritis. I. Prognostic value of IgA rheumatoid factor.早期类风湿性关节炎的前瞻性研究。I. IgA类风湿因子的预后价值。
Ann Rheum Dis. 1984 Oct;43(5):673-8. doi: 10.1136/ard.43.5.673.
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Use of monoclonal antibodies and F(ab')2 enzyme conjugates in ELISA for IgM, IgA and IgG rheumatoid factors.单克隆抗体和F(ab')2酶结合物在用于检测IgM、IgA和IgG类风湿因子的酶联免疫吸附测定（ELISA）中的应用。
J Immunol Methods. 1984 Jul 6;71(2):149-61. doi: 10.1016/0022-1759(84)90061-9.
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Measurement of rheumatoid factors by an enzyme-linked immunosorbent assay (ELISA) and comparison with other methods.通过酶联免疫吸附测定法（ELISA）检测类风湿因子并与其他方法进行比较。
Ann Rheum Dis. 1985 Jan;44(1):13-9. doi: 10.1136/ard.44.1.13.
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Laboratory diagnosis of rheumatoid arthritis: a solid phase radioassay for IgG and IgM antiglobulins.类风湿性关节炎的实验室诊断：一种检测IgG和IgM抗球蛋白的固相放射分析方法。
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Br Med J. 1975 Jul 26;3(5977):203-4. doi: 10.1136/bmj.3.5977.203.
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A sandwich method of enzyme-immunoassay. II. Quantification of rheumatoid factor.酶免疫测定的夹心方法。II. 类风湿因子的定量分析。
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