Pinchuk G V
Eksp Onkol. 1984;6(1):69-70.
In order to choose an optimal assay for screening cell microcultures containing anti-xenogenic erythrocyte antibodies in their supernatants, two microassays were compared: microhemagglutination and hemolytic patch assay. Samples of murine sera, each in both assays, were examined for the antibody titer. It is shown that the sensitivity of the assays is approximately at the same level, as the statistically significant difference in the amount of antibodies evaluated either by hemagglutination or by patch assay was not found. Hence one should not prefer any of the assays from the standpoint of sensitivity, when choosing the screening procedure for hybridomas of lymphoid cell microcultures secreting antibodies against xenogenic red blood cells.
为了选择一种最佳检测方法来筛选上清液中含有抗异种红细胞抗体的细胞微培养物，对两种微量检测方法进行了比较：微量血凝试验和溶血斑试验。在这两种检测方法中，对小鼠血清样本的抗体滴度进行了检测。结果表明，两种检测方法的灵敏度大致相同，因为通过血凝试验或溶血斑试验评估的抗体量没有发现统计学上的显著差异。因此，在选择针对异种红细胞分泌抗体的淋巴样细胞微培养物杂交瘤的筛选程序时，从灵敏度的角度来看，不应偏向任何一种检测方法。
