Brückner H, Graf H, Bokel M
Experientia. 1984 Nov 15;40(11):1189-97. doi: 10.1007/BF01946646.
Paracelsin, a hemolytic and membrane active polypeptide antibiotic of the peptaibol class which is excreted by the mold Trichoderma reesei, was obtained by a simplified and rapid isolation procedure utilizing hydrophobic adsorber resins. Investigation by 13C nuclear magnetic resonance spectroscopy and circular dichroism revealed considerable helical portions in solution, and the very recently accomplished sequence determination of paracelsin allows the discussion of the results with regard to the closely related analogues, alamethicin and suzukacillin. A selective cleavage of the peptide was achieved by careful treatment with various acids, and a buffer of pH 8.25 and of high ionic strength made possible the quantitative determination of the C-terminal phenylalaninol released by means of ion-exchange chromatography. The significance of the production of paracelsin and related mycotoxins of the peptaibol class, exhibiting various kinds of biological activity, is discussed with respect to the extensive effort being made towards biotechnological applications of species, strains and cellulolytically highly active mutants of the fungus Trichoderma.
副克列星是一种由里氏木霉分泌的肽菌素类溶血和膜活性多肽抗生素，通过使用疏水吸附树脂的简化快速分离程序获得。通过13C核磁共振光谱和圆二色性研究表明，该物质在溶液中有相当大的螺旋部分，最近完成的副克列星序列测定使得能够与密切相关的类似物阿拉霉素和铃木青霉素进行结果讨论。通过用各种酸仔细处理实现了肽的选择性裂解，pH 8.25和高离子强度的缓冲液使得通过离子交换色谱法定量测定释放的C末端苯丙氨醇成为可能。鉴于人们正在为木霉属真菌的物种、菌株和纤维素分解活性高的突变体的生物技术应用付出巨大努力，讨论了副克列星及相关肽菌素类霉菌毒素产生的意义，这些霉菌毒素具有各种生物活性。
