Bazafkan Hoda, Dattenböck Christoph, Böhmdorfer Stefan, Tisch Doris, Stappler Eva, Schmoll Monika
Department Health and Environment, AIT Austrian Institute of Technology GmbH, Bioresources, Konrad-Lorenz Strasse 24, Tulln, 3430, Austria.
University of Natural Resources and Life Sciences Vienna, Department of Chemistry, Division of Chemistry of Renewable Resources, Konrad-Lorenz-Straße 24, Tulln, 3430, Austria.
Mol Microbiol. 2015 Jun;96(6):1103-18. doi: 10.1111/mmi.12993. Epub 2015 Apr 16.
Sexual development in the filamentous model ascomycete Trichoderma reesei (syn. Hypocrea jecorina) was described only a few years ago. In this study, we show a novel role for VELVET in fungi, which links light response, development and secondary metabolism. Vel1 is required for mating in darkness, normal growth and conidiation. In light, vel1 was dispensable for male fertility but essential for female fertility in both mating types. VEL1 impacted regulation of the pheromone system (hpr1, hpr2, hpp1, ppg1) in a mating type-dependent manner and depending on the mating partner of a given strain. These partner effects only occurred for hpp1 and hpr2, the pheromone precursor and receptor genes associated with the MAT1-2 mating type and for the mating type gene mat1-2-1. Analysis of secondary metabolite patterns secreted by wild type and mutants under asexual and sexual conditions revealed that even in the wild type, the patterns change upon encounter of a mating partner, with again distinct differences for wild type and vel1 mutants. Hence, T. reesei applies a language of pheromones and secondary metabolites to communicate with mating partners and that this communication is at least in part mediated by VEL1.
丝状子囊菌里氏木霉（Trichoderma reesei，同义词为产黄青霉Hypocrea jecorina）的有性发育直到几年前才被描述。在本研究中，我们展示了VELVET在真菌中的一种新作用，它将光反应、发育和次级代谢联系起来。Vel1是黑暗中交配、正常生长和分生孢子形成所必需的。在光照条件下，vel1对于雄性育性并非必需，但对于两种交配类型的雌性育性却是必不可少的。VEL1以交配型依赖的方式并取决于给定菌株的交配伙伴，影响信息素系统（hpr1、hpr2、hpp1、ppg1）的调控。这些伙伴效应仅在hpp1和hpr2（与MAT1-2交配型相关的信息素前体和受体基因）以及交配型基因mat1-2-1中出现。对野生型和突变体在无性和有性条件下分泌的次级代谢产物模式的分析表明，即使在野生型中，遇到交配伙伴时模式也会发生变化，野生型和vel1突变体之间再次出现明显差异。因此，里氏木霉运用信息素和次级代谢产物的语言与交配伙伴进行交流，并且这种交流至少部分是由VEL1介导的。
