Schindler J, Hollingsworth R, Coughlin P
Differentiation. 1984;27(3):236-42. doi: 10.1111/j.1432-0436.1984.tb01434.x.
In monolayer cultures, embryonal carcinoma (EC) cells from the cell line Nulli-SCCl can be induced to differentiate at high efficiency by exposure to either retinoic acid or hexamethylenebisacetamide. Depending on which inducing agent is used, two distinct differentiated phenotypes result. These phenotypes resemble two of the earliest differentiated derivatives of the cells of the inner cell mass of a mouse blastocyst, i.e., parietal and visceral endoderm. Both differ from EC cells as well as from each other on the basis of their morphology, antigenic expression, secretion of plasminogen activator, and protein-synthetic profiles.
在单层培养中，来自Nulli-SCC1细胞系的胚胎癌细胞（EC）通过暴露于视黄酸或六甲撑双乙酰胺可被高效诱导分化。根据所使用的诱导剂不同，会产生两种不同的分化表型。这些表型类似于小鼠囊胚内细胞团细胞最早分化的两种衍生物，即滋养层和脏内胚层。二者在形态、抗原表达、纤溶酶原激活物分泌以及蛋白质合成谱方面均与EC细胞不同，且彼此也不同。
