Sénéchal H, Wahrmann J P, Delain D, Macieira-Coelho A
In Vitro. 1984 Sep;20(9):692-8. doi: 10.1007/BF02618874.
We examined the influence of attachment and spreading on myogenesis by adding polylysine-covered beads at different times after plating the cells on a plastic substratum. We show that polylysine per se acting on the cell surface can modulate myogenesis independently of cell spreading. Thus cell shape would not be the limiting factor for the division and differentiation of L6 myoblasts. Multinucleation of the cells was found to be first enhanced by the addition of polylysine-covered beads to replicating myoblasts, although the final percentage of fusion attained by these cultures was lower than in the controls. A similar phenomenon was observed concerning myosin synthesis. No such effect could be observed when the beads were added to a nonfusing mutant or to fibroblasts. Our results show that this phenomenon is specific. We postulate that some of the surface molecules necessary for this process appear on myoblasts shortly before they fuse.
我们通过在将细胞接种到塑料基质上后的不同时间添加聚赖氨酸包被的珠子，研究了黏附与铺展对肌生成的影响。我们发现，作用于细胞表面的聚赖氨酸本身可独立于细胞铺展来调节肌生成。因此，细胞形状并非L6成肌细胞分裂与分化的限制因素。尽管这些培养物最终达到的融合百分比低于对照组，但在增殖的成肌细胞中添加聚赖氨酸包被的珠子会首先增强细胞的多核化。在肌球蛋白合成方面也观察到了类似现象。当将珠子添加到非融合突变体或成纤维细胞中时，未观察到这种效应。我们的结果表明这种现象具有特异性。我们推测，这一过程所需的一些表面分子在成肌细胞融合前不久出现在其表面。
